Directed termination PCR: A one-step approach to mutation detection
نویسندگان
چکیده
منابع مشابه
Directed termination PCR: a one-step approach to mutation detection.
We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or non-denaturing polyacrylamide gels. This method provides a one-step and highly effective approach for t...
متن کاملMutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection.
With increasing knowledge about the causal role of genetic defects in clinical diseases the necessity is apparent to have procedures for rapid diagnosis of point mutations. We developed a PCR-based technique, whereby both normal and mutant alleles can be amplified in the same reaction tube, using different length allele-specific primers. Furthermore the allele-specific primers introduce additio...
متن کاملFluorescence-based directed termination PCR: direct mutation characterization without sequencing.
We describe a fluorescence-based directed termination PCR (fluorescent DT-PCR) that allows accurate determination of actual sequence changes without dideoxy DNA sequencing. This is achieved using near infrared dye-labeled primers and performing two PCR reactions under low and unbalanced dNTP concentrations. Visualization of resulting termination fragments is accomplished with a dual dye Li-cor ...
متن کاملAspergillus PCR: one step closer to standardization.
PCR has been used as an aid in the diagnosis of invasive aspergillosis for almost 2 decades. A lack of standardization has limited both its acceptance as a diagnostic tool and multicenter clinical evaluations, preventing its inclusion in disease-defining criteria. In 2006, the European Aspergillus PCR Initiative was formed. The aim of the initiative was to provide optimal standardized protocols...
متن کاملA Step Size Preserving Directed Mutation Operator
The main idea of the directed mutation is to focus on mutating into the most beneficial direction by using a customizable asymmetrical distribution. In this way the optimization strategy can adopt the most promising mutation direction over the generations. It thus becomes nearly as flexible as with Schwefel’s correlated mutation [2] but causes only linear growth of the strategy parameters inste...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/26.6.1546